RESUMO
BACKGROUND: Collective and discrete neural crest cell (NCC) migratory streams are crucial to vertebrate head patterning. However, the factors that confine NCC trajectories and promote collective cell migration remain unclear. RESULTS: Computational simulations predicted that confinement is required only along the initial one-third of the cranial NCC migratory pathway. This guided our study of Colec12 (Collectin-12, a transmembrane scavenger receptor C-type lectin) and Trail (tumor necrosis factor-related apoptosis-inducing ligand, CD253) which we show expressed in chick cranial NCC-free zones. NCC trajectories are confined by Colec12 or Trail protein stripes in vitro and show significant and distinct changes in cell morphology and dynamic migratory characteristics when cocultured with either protein. Gain- or loss-of-function of either factor or in combination enhanced NCC confinement or diverted cell trajectories as observed in vivo with three-dimensional confocal microscopy, respectively, resulting in disrupted collective migration. CONCLUSIONS: These data provide evidence for Colec12 and Trail as novel NCC microenvironmental factors playing a role to confine cranial NCC trajectories and promote collective cell migration.
Assuntos
Movimento Celular , Galinhas , Crista Neural , Animais , Diferenciação Celular/genética , Diferenciação Celular/fisiologia , Movimento Celular/genética , Movimento Celular/fisiologia , Galinhas/genética , Galinhas/fisiologia , Simulação por Computador , Crista Neural/citologia , Crista Neural/fisiologia , CrânioRESUMO
OBJECTIVE: Objective. To reflect on the process of committing to participation in the implementation of a health strategic plan, using Participative Systematization of Social Experiences as a tool. MATERIAL AND METHODS: Our study was a qualitative research-intervention study, based on the Dialectical Methodological Conception approach. We designed and implemented a two-day workshop, six hours daily, using Systematization methodology with a Community Work Group (CWG). During the workshop, women systematized their experience, with compromise as axis of the process. Using Grounded Theory techniques, we applied micro-analysis to data in order to identify and strengthen categories that emerged during the systematization process. We completed open and axial coding. RESULTS: The CWG identified that commitment and participation itself is influenced by group dynamics and structural determinants. They also reconsidered the way they understood and exercised commitment and participation, and generated knowledge, empowering them to improve their future practice. CONCLUSIONS: Commitment and participation were determined by group dynamics and structural factors such as socioeconomic conditions and gender roles. These determinants must be visible and understood in order to generate proposals that are aimed at strengthening the participation and organization of groups.
OBJETIVO: Reflexionar sobre el compromiso en el proceso de participación en salud empleando la herramienta de sistematización participativa de experiencias sociales. MATERIAL Y MÉTODOS: Investigación-intervención de corte cualitativo, desde la concepción metodológica dialéctica. Las mujeres pertenecientes a un grupo de trabajo comunitario en Xoxocotla, Morelos, México, sistematizaron su experiencia utilizando como eje el compromiso. Con base en técnicas derivadas de la teoría fundamentada, se realizó un microanálisis para identificar y robustecer las categorías del contenido correspondiente al proceso de sistematización. RESULTADOS: Las participantes identificaron los determinantes estructurales del compromiso en su proceso de participación y elementos relacionados con la dinámica grupal que lo influyen; asimismo, redefinieron la forma de entender y ejercer el compromiso y la participación, y extrajeron aprendizajes para mejorar su práctica futura. CONCLUSIONES: El compromiso y la participación están determinados por factores estructurales como la condición socioeconómica y el rol de género, los cuales deben visibilizarse y comprenderse para generar propuestas que fortalezcan el involucramiento y la organización de grupos.
Assuntos
Participação da Comunidade , Educação em Saúde , Conhecimentos, Atitudes e Prática em Saúde , Humanos , México , Fatores SociológicosRESUMO
Resumen: Objetivo: Reflexionar sobre el compromiso en el proceso de participación en salud empleando la herramienta de sistematización participativa de experiencias sociales. Material y métodos: Investigación-intervención de corte cualitativo, desde la concepción metodológica dialéctica. Las mujeres pertenecientes a un grupo de trabajo comunitario en Xoxocotla, Morelos, México, sistematizaron su experiencia utilizando como eje el compromiso. Con base en técnicas derivadas de la teoría fundamentada, se realizó un microanálisis para identificar y robustecer las categorías del contenido correspondiente al proceso de sistematización. Resultados: Las participantes identificaron los determinantes estructurales del compromiso en su proceso de participación y elementos relacionados con la dinámica grupal que lo influyen; asimismo, redefinieron la forma de entender y ejercer el compromiso y la participación, y extrajeron aprendizajes para mejorar su práctica futura. Conclusiones: El compromiso y la participación están determinados por factores estructurales como la condición socioeconómica y el rol de género, los cuales deben visibilizarse y comprenderse para generar propuestas que fortalezcan el involucramiento y la organización de grupos.
Abstract: Objective: To reflect on the process of committing to participation in the implementation of a health strategic plan, using Participative Systematization of Social Experiences as a tool. Materials and methods: Our study was a qualitative research-intervention study, based on the Dialectical Methodological Conception approach. We designed and implemented a two-day workshop, six hours daily, using Systematization methodology with a Community Work Group (CWG). During the workshop, women systematized their experience, with compromise as axis of the process. Using Grounded Theory techniques, we applied micro-analysis to data in order to identify and strengthen categories that emerged during the systematization process. We completed open and axial coding. Results: The CWG identified that commitment and participation itself is influenced by group dynamics and structural determinants. They also reconsidered the way they understood and exercised commitment and participation, and generated knowledge, empowering them to improve their future practice. Conclusions: Commitment and participation were determined by group dynamics and structural factors such as socioeconomic conditions and gender roles. These determinants must be visible and understood in order to generate proposals that are aimed at strengthening the participation and organization of groups.
